High purity Nile Red fluorescence dye (95.0% HPLC) for professional analysis. Reliable CAS 7385-67-3 chemical reagent from Mclean for lab applications.
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Ensure precision in your laboratory research with Macklin’s high-purity Nile Red. This specialized chemical reagent is meticulously synthesized for advanced fluorescence analysis, offering the reliability required for sensitive imaging and detection tasks. As a lipophilic stain, Nile Red is an essential tool for researchers in cell biology, material science, and environmental testing, particularly for the visualization of lipids and hydrophobic materials.
Nile Red is a hydrophobic dye and is typically insoluble in water. To prepare for use, dissolve the solid reagent in an appropriate organic solvent such as DMSO, ethanol, or acetone to create a concentrated stock solution. For fluorescence microscopy or flow cytometry, dilute the stock solution into your working buffer or medium.
When staining, apply the solution to the sample and allow for an incubation period as dictated by your specific protocol. Because this reagent is used for fluorescence analysis, a fluorescence microscope or spectrofluorometer is required for detection. Adjust excitation and emission settings based on the polarity of the environment, as Nile Red’s fluorescence properties shift depending on the hydrophobicity of its surroundings.
"Used for fluorescence analysis" to provide high-contrast imaging in various hydrophobic environments.
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Ensure precision in your laboratory research with Macklin’s high-purity Nile Red. This specialized chemical reagent is meticulously synthesized for advanced fluorescence analysis, offering the reliability required for sensitive imaging and detection tasks. As a lipophilic stain, Nile Red is an essential tool for researchers in cell biology, material science, and environmental testing, particularly for the visualization of lipids and hydrophobic materials.
Nile Red is a hydrophobic dye and is typically insoluble in water. To prepare for use, dissolve the solid reagent in an appropriate organic solvent such as DMSO, ethanol, or acetone to create a concentrated stock solution. For fluorescence microscopy or flow cytometry, dilute the stock solution into your working buffer or medium.
When staining, apply the solution to the sample and allow for an incubation period as dictated by your specific protocol. Because this reagent is used for fluorescence analysis, a fluorescence microscope or spectrofluorometer is required for detection. Adjust excitation and emission settings based on the polarity of the environment, as Nile Red’s fluorescence properties shift depending on the hydrophobicity of its surroundings.
"Used for fluorescence analysis" to provide high-contrast imaging in various hydrophobic environments.